Isoenzimi I e II della 5 alfa reduttasi presenti nella prostata umana: studio comparativo riguardante diversi inibitori selettivi(finasteride,saw palmetto,turosteride)
Author: Iehlé C; Délos S; Guirou O; Tate R; Raynaud JP; Martin PM
Address: Laboratoire de Cancérologie Expérimentale, Faculté de Médecine, Marseille, France.
Source: J Steroid Biochem Mol Biol, 54: 5-6, 1995 Sep, 273-9
The present study describes the independent expression of the type 1 and 2 isoforms of human 5 alpha-reductase in the baculovirus-directed insect cell expression system and the selectivity of their inhibition. The catalytic properties and kinetic parameters of the recombinant isozymes were consistent with published data.
The type 1 isoform displayed a neutral (range 6-8) pH optimum and the type 2 isoform an acidic (5-6) pH optimum. The type 2 isoform had higher affinity for testosterone than did the type 1 isoform (Km = 0.5 and 2.9 microM, respectively).
Finasteride and turosteride were selective inhibitors of the type 2 isoform (Ki (type 2) = 7.3 and 21.7 nM compared to Ki (type 1) = 108 and 330 nM, respectively). 4-MA and the lipido-sterol
extract of Serenoa repens (LSESr) markedly inhibited both isozymes (Ki(type1) = 8.4 nM and 7.2 micrograms/ml, respectively; Ki (type 2) = 7.4 nM and 4.9 micrograms/ml, respectively). The three azasteroids were competitive inhibitors vs substrate, whereas LSESr displayed non-competitive inhibition of the type 1 isozyme and uncompetitive inhibition of the type 2 isozyme.
These observations suggest that the lipid component of LSESr might be responsible for its inhibitory effect by modulating the membrane environment of 5 alpha-reductase.
Partially purified recombinant 5 alpha-reductase type 1 activity was preserved by the presence of lipids indicating that lipids can exert either stimulatory or inhibitory effects on human 5 alpha-reductase.
Language of Publication English – Unique Identifier 96042333