Non so se può essere utile... In rete ho trovato questo, estratto dal GEBIN 2017 - 12th Scientific Meeting - March 23 – 25, 2017 - Münster | Germany
Link al documento https://www.uni-muenster.de/imperia/md/content/gebin-2017/gebin_2017_meeting_booklet.pdf
OLFACTORY RECEPTOR STIMULATION PROMOTES HUMAN HAIR GROWTH
Chemical signals acting on olfactory receptors (OR) regulate many cell functions beyond olfaction, e.g. OR2AT4-mediated keratinocyte (kerat) proliferation and migration in vitro. Here, we asked whether OR2AT4 also plays a role in human hair follicle (hHF) biology. Indeed, anagen scalp hHFs prominently express OR2AT4 protein (IF) in the central outer root sheath, and up-regulate OR2AT4 transcription (qRT-PCR) after stimulation with a specific OR2AT4 agonist, Sandalore®. Sandalore® significantly retarded spontaneous catagen development in anagen HFs ex vivo and decreased the number of apoptotic (TUNEL+ or caspase-3+)hair matrix (HM) kerat compared to controls. These effects were partially counteracted by coadministering the specific OR2AT4 antagonist, Phenirat®. Most importantly, catagen was induced prematurely and HM kerat apoptosis was en hanced in OR2AT4-silenced hHFs, compared to vehicle HFs, even under Sandalore® stimulation. Sandalore® significantly decreased TGFß2 expression, but significantly increased IGF-1. OR2AT4 knock-down decreased IGF-1 expression while TGFß2 expression was unchanged, suggesting that OR2AT4 activation impacts on HF cycling via up-regulating IGF-1. Microarray analysis showed that Sandalore® significantly downregulated the transcription of pro-apoptotic and up-regulated anti -apoptotic genes. By phosphokinase assay, phosphorylation of kinases involved in the IGF-1 pathway was enhanced under Sandalore® stimulation. In summary, we show for the first time that hHFs engage in OR-mediated chemosensation and that OR2AT4 stimulation is required to maintain hHFs in anagen ex vivo. This suggests that OR2AT4 ligands like Sandalore® can be recruited for the management of hHF growth disorders.
