Notizia passata sotto silenzion ma del massimo interesse.
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J Invest Dermatol. 2007 May 17; [Epub ahead of print] Links
Androgen Receptor Co-Activator Hic-5/ARA55 as a Molecular Regulator of Androgen Sensitivity in Dermal Papilla Cells of Human Hair Follicles.Inui S, Fukuzato Y, Nakajima T, Kurata S, Itami S.
1Department of Regenerative Dermatology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Androgen site-specifically affects human hair growth after puberty through androgen receptors in the dermal papilla, which transactivate target genes acting in conjunction with co-activators. To examine the regulation of androgen sensitivity in hair follicles, we focused on androgen receptor co-activator Hic-5/ARA55. Its interaction with transfected androgen receptor in beard dermal papilla cells was confirmed with mammalian two-hybrid assays. The semiquantitative reverse transcriptase-polymerase chain reaction showed that Hic-5/ARA55 mRNA expression was high in dermal papilla cells from the beard and bald frontal scalp but low in cells from the occipital scalp. To determine whether Hic-5/ARA55 mRNA level correlates with its endogenous activity, we studied the effect of dominant negative Hic-5/ARA55 on transfected androgen receptor transactivation induced by R1881 using mouse mammary tumor virus-luciferase assays. We found that it suppressed the transactivation by 64.5 and 71.4% in dermal papilla cells from the beard and bald frontal scalp, respectively, whereas it showed no significant effect in cells from the occipital scalp. Our findings suggest that Hic-5/ARA55 is a molecular regulator for androgen sensitivity in human hair follicles.Journal of Investigative Dermatology advance online publication, 17 May 2007; doi:10.1038/sj.jid.5700883.
PMID: 17508020 [PubMed - as supplied by publisher]
mi sembra del massimo interessen nella misura in cui le regione frontale e occipitale non sempre rispondo allo stesso modo. La questione potrebbe coinvolgere la differente sensibilità mediata da questo coattivatore - oltre che, s'intende, al polimorfismo del recettore AR - che sarebbe molto attivo nella zona frontale e assente in quella occipitale. Fra gli autori della ricerca il leggendario Inui, uno dei massimi esperti internazionali nella ricerca tricologica (sono celebri i suoi studi sul minoxidil).
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J Invest Dermatol. 2007 May 17; [Epub ahead of print] Links
Androgen Receptor Co-Activator Hic-5/ARA55 as a Molecular Regulator of Androgen Sensitivity in Dermal Papilla Cells of Human Hair Follicles.Inui S, Fukuzato Y, Nakajima T, Kurata S, Itami S.
1Department of Regenerative Dermatology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Androgen site-specifically affects human hair growth after puberty through androgen receptors in the dermal papilla, which transactivate target genes acting in conjunction with co-activators. To examine the regulation of androgen sensitivity in hair follicles, we focused on androgen receptor co-activator Hic-5/ARA55. Its interaction with transfected androgen receptor in beard dermal papilla cells was confirmed with mammalian two-hybrid assays. The semiquantitative reverse transcriptase-polymerase chain reaction showed that Hic-5/ARA55 mRNA expression was high in dermal papilla cells from the beard and bald frontal scalp but low in cells from the occipital scalp. To determine whether Hic-5/ARA55 mRNA level correlates with its endogenous activity, we studied the effect of dominant negative Hic-5/ARA55 on transfected androgen receptor transactivation induced by R1881 using mouse mammary tumor virus-luciferase assays. We found that it suppressed the transactivation by 64.5 and 71.4% in dermal papilla cells from the beard and bald frontal scalp, respectively, whereas it showed no significant effect in cells from the occipital scalp. Our findings suggest that Hic-5/ARA55 is a molecular regulator for androgen sensitivity in human hair follicles.Journal of Investigative Dermatology advance online publication, 17 May 2007; doi:10.1038/sj.jid.5700883.
PMID: 17508020 [PubMed - as supplied by publisher]
mi sembra del massimo interessen nella misura in cui le regione frontale e occipitale non sempre rispondo allo stesso modo. La questione potrebbe coinvolgere la differente sensibilità mediata da questo coattivatore - oltre che, s'intende, al polimorfismo del recettore AR - che sarebbe molto attivo nella zona frontale e assente in quella occipitale. Fra gli autori della ricerca il leggendario Inui, uno dei massimi esperti internazionali nella ricerca tricologica (sono celebri i suoi studi sul minoxidil).
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