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]Hair growth properties discovered in plant extract
J Dermatol Sci. 2005 May;38(2):89-97. Epub 2005 Mar 19.
The hair growth promoting effect of Asiasari radix extract and its molecular regulation.
Rho SS, Park SJ, Hwang SL, Lee MH, Kim CD, Lee IH, Chang SY, Rang MJ.
Oriental Medical College of Daejeon University, 22-5 Daeheung-dong, Daejeon 301-724, South Korea.
BACKGROUND:: Hair loss is a distressing condition for an increasing number of men and women. It is of great importance; therefore, to develop new therapies for the treatment of hair loss.
OBJECTIVE:: We examined the effects of 45 plant extracts that have been traditionally used for treating hair loss in oriental medicine in order to identify potential stimulants of hair growth.
METHODS:: Six-week-old female C57BL/6 and C3H mice were used for evaluating the hair growth-promoting effects of the plant extracts. Topical application onto the backs of the C57BL/6 and C3H mice was performed daily for 30 days and 45 days, respectively. Protein synthesis was measured by the cysteine uptake assay, using cultured murine vibrissae follicles. Proliferation of the immortalized human keratinocyte cell line (HaCaT) and human dermal papilla (DP) cells was evaluated by the MTT and thymidine incorporation assays. The mRNA levels of several growth factors that have been implicated in hair growth control were measured by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS:: Among the tested plant extracts, the extract of Asiasari radix showed the most potent hair growth stimulation in C57BL/6 and C3H mice experiments. In addition, this extract markedly increased the protein synthesis in vibrissae follicle cultures and the proliferation of both HaCaT and human DP cells in vitro. Moreover, the A. radix extract induced the expression of VEGF in human DP cells that were cultured in vitro.
CONCLUSION:: These results suggest that the A. radix extract has hair growth-promoting potential, and that this effect may be due to its regulatory effects on both cell growth and growth factor gene expression.
STUDY SUMMARY
The Asiasari radix plant
This is a South Korean study that set out to examine different plant extracts used to treat hair loss in traditional oriental medicine. Of the 45 plant extracts one stood out as having more potential to stimulate hair growth than the others – The Asiasari radix, also known as Asiasarum root.
Female mice were used in the study, the backs of the mice were shaved and a topical solution of 1% Asiasari radix extract in 40% ethanol was applied for 30 days, the control group received 40% ethanol only. At the time of the first application all mice hair follicles were in telogen (resting) state and the criteria for hair growth promotion was an earlier telogen to anagen (growing) conversion in the treatment group than in the control group. This criteria was clearly met in the first 30 day study and again in a following 45 day study in a different line of mice. A closer examination of the follicles showed that they had increased in both depth and size in the A. radix treated mice compared to the control.
Follicles from the mice whiskers were taken, cultured and subjected to A. radix extract, after which the protein synthesis was measured by cysteine uptake. Cysteine is an amino acid and used in protein synthesis and cysteine uptake is known to correlate with hair follicle growth in vitro. The uptake was higher in the A. radix group than in the control, indicating increased follicle growth.
Tests on how A. radix extract affects human cells were also done, using human keratinocytes and dermal papilla cells. Keratinocytes produce keratin, the main component of hair and dermal papilla cells are located in the deepest end of the hair follicle and is believed to be important for hair growth and induction of new hair follicles. The results showed that A. radix increased the proliferation of both types of cells compared to control.
In addition to this they also studied how the A. radix extract influenced different growth factors shown to effect hair growth. In the keratinocytes there was no significant difference compared to control but in the dermal papilla cells there was a significant increase in expression of vascular endothelial growth factor (VEGF), a growth factor involved in forming new blood vessels. The expression of VEGF is normally increased in dermal papilla cells during anagen phase and then decreased in the telogen and catagen phases. Minoxidil also increase the expression of VEGF and one theory is that it’s through this that it stimulates the follicles to remain in anagen phase.
The researchers also examined if the A. radix extract had any influence on 5-alpha reductase, the enzyme that converts testosterone to DHT, but they found no evidence of that. The conclusion of the study was that A. radix promotes hair growth in mice and that it promotes cell proliferation and VEGF expression in human cells as well, indicating that it may also have hair growing properties in humans.
Link:http://www.hairlosshelp.com/hair_loss_news/index.cfm?CommentID=26
Basilio
J Dermatol Sci. 2005 May;38(2):89-97. Epub 2005 Mar 19.
The hair growth promoting effect of Asiasari radix extract and its molecular regulation.
Rho SS, Park SJ, Hwang SL, Lee MH, Kim CD, Lee IH, Chang SY, Rang MJ.
Oriental Medical College of Daejeon University, 22-5 Daeheung-dong, Daejeon 301-724, South Korea.
BACKGROUND:: Hair loss is a distressing condition for an increasing number of men and women. It is of great importance; therefore, to develop new therapies for the treatment of hair loss.
OBJECTIVE:: We examined the effects of 45 plant extracts that have been traditionally used for treating hair loss in oriental medicine in order to identify potential stimulants of hair growth.
METHODS:: Six-week-old female C57BL/6 and C3H mice were used for evaluating the hair growth-promoting effects of the plant extracts. Topical application onto the backs of the C57BL/6 and C3H mice was performed daily for 30 days and 45 days, respectively. Protein synthesis was measured by the cysteine uptake assay, using cultured murine vibrissae follicles. Proliferation of the immortalized human keratinocyte cell line (HaCaT) and human dermal papilla (DP) cells was evaluated by the MTT and thymidine incorporation assays. The mRNA levels of several growth factors that have been implicated in hair growth control were measured by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS:: Among the tested plant extracts, the extract of Asiasari radix showed the most potent hair growth stimulation in C57BL/6 and C3H mice experiments. In addition, this extract markedly increased the protein synthesis in vibrissae follicle cultures and the proliferation of both HaCaT and human DP cells in vitro. Moreover, the A. radix extract induced the expression of VEGF in human DP cells that were cultured in vitro.
CONCLUSION:: These results suggest that the A. radix extract has hair growth-promoting potential, and that this effect may be due to its regulatory effects on both cell growth and growth factor gene expression.
STUDY SUMMARY
The Asiasari radix plant
This is a South Korean study that set out to examine different plant extracts used to treat hair loss in traditional oriental medicine. Of the 45 plant extracts one stood out as having more potential to stimulate hair growth than the others – The Asiasari radix, also known as Asiasarum root.
Female mice were used in the study, the backs of the mice were shaved and a topical solution of 1% Asiasari radix extract in 40% ethanol was applied for 30 days, the control group received 40% ethanol only. At the time of the first application all mice hair follicles were in telogen (resting) state and the criteria for hair growth promotion was an earlier telogen to anagen (growing) conversion in the treatment group than in the control group. This criteria was clearly met in the first 30 day study and again in a following 45 day study in a different line of mice. A closer examination of the follicles showed that they had increased in both depth and size in the A. radix treated mice compared to the control.
Follicles from the mice whiskers were taken, cultured and subjected to A. radix extract, after which the protein synthesis was measured by cysteine uptake. Cysteine is an amino acid and used in protein synthesis and cysteine uptake is known to correlate with hair follicle growth in vitro. The uptake was higher in the A. radix group than in the control, indicating increased follicle growth.
Tests on how A. radix extract affects human cells were also done, using human keratinocytes and dermal papilla cells. Keratinocytes produce keratin, the main component of hair and dermal papilla cells are located in the deepest end of the hair follicle and is believed to be important for hair growth and induction of new hair follicles. The results showed that A. radix increased the proliferation of both types of cells compared to control.
In addition to this they also studied how the A. radix extract influenced different growth factors shown to effect hair growth. In the keratinocytes there was no significant difference compared to control but in the dermal papilla cells there was a significant increase in expression of vascular endothelial growth factor (VEGF), a growth factor involved in forming new blood vessels. The expression of VEGF is normally increased in dermal papilla cells during anagen phase and then decreased in the telogen and catagen phases. Minoxidil also increase the expression of VEGF and one theory is that it’s through this that it stimulates the follicles to remain in anagen phase.
The researchers also examined if the A. radix extract had any influence on 5-alpha reductase, the enzyme that converts testosterone to DHT, but they found no evidence of that. The conclusion of the study was that A. radix promotes hair growth in mice and that it promotes cell proliferation and VEGF expression in human cells as well, indicating that it may also have hair growing properties in humans.
Link:http://www.hairlosshelp.com/hair_loss_news/index.cfm?CommentID=26
Basilio